12th class (Fsc) Biology unit 9 MCQs
chapter 9 MCQs 12th Class Biology
Biotechnology:
1. Which of these is incorrectly matched?
a. DNA polymerase- PCR
b. Protoplast plant cell engineering
c. DNA ligase mapping human chromosomes
d. RFLPS –DNA finger printing
2. Which of these is a true statement?
a. Vectors carry only the foreign gene into the host cell
b. Both plasmids and virus can serve as vectors
c. Only gene therapy uses vectors
d. Plasmids can carry recombinant DNA but viruses can not
3. Which of these is a benefit to having insulin produced by biotechnology?
a. It is nonallergenic
b. It is just as effective
c. It is less expensive
d. It can be mass produced
4. Which of these would you not expect to be a biotechnology product?
a. DNA probes
b. Vaccine
c. Protein hormones
d. Modified enzyme
5. The polymerase chain reaction (PCR) was developed in 1983 by:
a. Theodore M. Klein
b. Kary B.Mullis
c. Craig Venter
d. Gottlieb Haberlandt
6. PCR takes its name from the enzyme that carries out DNA replication in a cell:
a. DNA ligase
b. DNA polymerase
c. Restriction enzymes
d. DNA polymerase II
7. The enzymes which are used to cut out the gene of interest are known as:
a. RNA polymerase
b. DNA ligase
c. Restriction endonuclease
d. DNA polymerase
8. Recombinant DNA is introduced into the host cell by means of a:
a. Bacterium
b. Vector
c. Fungus
d. Phage
9. The enzyme which joins the two pieces of DNA is:
a. Restriction endonuclease
b. DNA polymerase-I
c. DNA polymerase-I
d. DNA ligase
10. The two different pieces of DNA joined together is called as:
a. Trimeric DNA
b. Dimeric DNA
c. Tetrameric DNA
d. Chimaeric DNA
11. Eco R1 is a commonly used:
a. Bacteriophage
b. Gene
c. Bacteria
d. Restriction enzyme
12. PBR 322 has antibiotic resistant gene for:
a. Ampicillin
b. Tetracycline and ampicillin
c. Streptomycin only
d. Tetracycline only
13. PSC 101 has antibiotic resistant genes for:
a. Nemocyin
b. Tetracycline
c. Ergotine
d. Ampicillin
14. Which is not a biotechnology product?
a. Human growth hormone
b. Hepatitis B
c. Hemophilia factor I
d. Tissue plasminogen activator
15. A collection of bacterial and phage viruses clones containing a particular segment of DNA from the source cell is called:
a. Genomic library
b. Recombinant DNA
c. Genome
d. Expressing system
16. Hamilton O smith isolated the first restriction enzyme in:
a. 1970
b. 1950
c. 1980
d. 1960
17. First restriction enzyme was isolated by:
a. Sanger
b. Kary Mullis
c. Mendel
d. Hamilton
18. The first restriction enzyme was isolated by:
a. Sanger
b. Kary Mullis
c. Maxam Gilbert
d. Hamilton O.Smith
19. Thermos aquaticus is a/an:
a. Alga
b. Fungus
c. Bacterium
d. Protozoan
20. Kary B.Mullis developed the polymerase chain reaction in:
a. 1983
b. 1963
c. 1993
d. 1973
21. A probe is s single stranded nucleotide sequence that will hybridize in to certain piece of:
a. DNA
b. RNA
c. Amino acids
d. Carbohydrate
22. Taq polymerase are enzyme present in:
a. Algae
b. Fungi
c. Virus
d. Bacteria
23. Restriction fragment length polymorphism (RFLPs):
a. Are the base for DNA finger prints
b. Are achieved by using restriction enzymes
c. Can be subjected to gel electrophoresis
d. Identify individuals genetically
24. Gel electrophoresis:
a. Tells whether viruses are infectious
b. Cannot be used on nucleotides
c. Measure the change and size of proteins and DNA fragments
d. Measures the size of plasmids
25. Cases of disputed parenthood can be solved with the help of:
a. Cloning
b. DNA finger printing
c. Gene sequencing
d. RNA finger Printing